Regulatory

Part:BBa_K3428003:Design

Designed by: Andrew Sillato   Group: iGEM20_Stony_Brook   (2020-10-26)


UVB Light Responsive Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 120


Design Notes

The 35s CaMV promoter is a strong constitutive promoter in plants, but when reduced to its core, transcription of genes is only permitted under the effects of the binding of a downstream activator. Truncation of the 35s CaMV promoter to its core was therefore performed. The core promoter is here defined as the region of the 35s CaMV promoter between the transcription start site, and the point 15 nucleotides upstream from the TATA box of the promoter.

Note that this part did not undergo real-world characterization due to COVID-19 restrictions



Source

tetR operator sequence (tetO)- BBa_K105020 35s CaMV Promoter - BBa_K1825004

References

[1] Motta-Mena, L., Reade, A., Mallory, M. et al. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat Chem Biol 10, 196–202 (2014). https://doi.org/10.1038/nchembio.1430